GrossingGrossing is the process by which a tissue sample is removed from its fixative and placed in a labeled cassette. Each piece of tissue has a designated cassette for individual identification.
DecalcificationDecalcification is the process by which calcium is removed from bone, or other hard tissues (at the technician's discretion), before processing.
ProcessingProcessing involves the dehydration, clearing, and infiltration of paraffin to prepare the tissues for embedding.
EmbeddingEmbedding involves enclosing the tissue in solidified paraffin wax, which provides the block to be prepared for microtomy.
MicrotomyMicrotomy is the process by which paraffin tissue blocks are cut into thin sections using a microtome and placed on slides for further examination under a microscope. The common thickness for sections is 5 microns.
- Level 1
- Alcian Blue: demonstrates acid mucopolysaccharides
- Colloidal Iron: demonstrates carboxylated and sulfated mucopolysaccarides and glycoproteins in tissue
- Congo Red: demonstrates amyloid deposits in tissue
- Giemsa: demonstrates bacteria and rickettsias in tissue
- Kinyoun's AFB: demonstrates the presence of acid fast mycobacteria in tissue
- Luxol Fast Blue: demonstrates myelin and Nissl substance in tissue
- Mayer's Mucicarmine: staining of "epithelial" mucin in tissue
- PAS w/o digestion: demonstrates polysaccharides, neutral mucosubstances & basement membranes
- Saffron O: demonstrates the detection of cartilage
- Toluidine Blue: demonstrates the presence of mast cells in tissue
- Level 2
- Gram: demonstrates gram-negative and gram-positive in tissue
- Masson's Trichrome: demonstrates the differentiation between collagen and smooth muscle in tumors and identify increases in collagenous tissue in diseases
- Oil Red O: demonstrates neutral lipids in frozen tissue section; can only be performed on frozen tissue sections
- PAS /AB: used to differentiate between neutral & acidic mucosubstances
- PAS w/digestion: demonstrates the presence of glycogen in tissue
- Prussian Blue: demonstrates the detection of ferric iron in tissue
- Verhoeff's: demonstrates pathologic changes in elastic fibers of tissue
- Level 3
- Sevier-Munger: demonstrates nerve fibers and the presence of neurofibrillary tangles and senile plaques in Alzheimer's disease
- Grocott's Methenamine Silver (GMS): demonstrates the presence of fungal organisms and pneumocystis
- Von Kossa's: demonstrates the presence of calcium in tissue
DNA Slide PreparationThere is an additional charge for handling DNA projects, a new blade is used for each block to eliminate any cross contamination. The charge is $3.00 per blade.
Frozen SectionsWe accept frozen tissue samples mounted in OCT and properly labeled with the specimen identification.
It is imperative that the tissues are received in the proper molds. If the tissue mold is not correct and does not fit our machine, we will return the samples to the Principal Investigator. If you have any questions concerning the mold, please call us in advance.
We will place two sections on one slide and there is a fee of $.75 for every additional section. The tissue samples are required to ship priority overnight in dry ice with proper dry ice handling procedure. Be sure to provide enough dry ice to last 48 hours; this is approximately 10 pounds of dry ice. DO NOT SHIP FROZEN SAMPLES ON A FRIDAY OR A HOLIDAY. Frozen sections need to be shipped Priority Overnight on dry ice. There is a dry ice charge of $25 when return shipping.